Human microcephaly ASPM protein is a spindle pole-focusing factor that functions redundantly with CDK5RAP2
نویسندگان
چکیده
منابع مشابه
Human microcephaly ASPM protein is a spindle pole-focusing factor that functions redundantly with CDK5RAP2.
Nonsense mutations in the ASPM gene have been most frequently identified among familial microcephaly patients. Depletion of the Drosophila orthologue (asp) causes spindle pole unfocusing during mitosis in multiple cell types. However, it remains unknown whether human ASPM has a similar function. Here, by performing CRISPR-based gene knockout (KO) and RNA interference combined with auxin-inducib...
متن کاملHuman microcephaly ASPM protein is a spindle pole-focusing factor
Nonsense mutations in the ASPM gene have been most frequently identified among familial microcephaly patients. Depletion of ASPM causes spindle pole unfocusing during mitosis in multiple cell types of Drosophila. However, it remains unknown whether human ASPM has a similar function. Here, using CRISPR-based gene knockout (KO) and RNA interference combined with chemical inhibitors and auxin-indu...
متن کاملThe microcephaly ASPM gene is expressed in proliferating tissues and encodes for a mitotic spindle protein.
The most common cause of primary autosomal recessive microcephaly (MCPH) appears to be mutations in the ASPM gene which is involved in the regulation of neurogenesis. The predicted gene product contains two putative N-terminal calponin-homology (CH) domains and a block of putative calmodulin-binding IQ domains common in actin binding cytoskeletal and signaling proteins. Previous studies in mous...
متن کاملCDK5RAP2 functions in centrosome to spindle pole attachment and DNA damage response
The centrosomal protein, CDK5RAP2, is mutated in primary microcephaly, a neurodevelopmental disorder characterized by reduced brain size. The Drosophila melanogaster homologue of CDK5RAP2, centrosomin (Cnn), maintains the pericentriolar matrix (PCM) around centrioles during mitosis. In this study, we demonstrate a similar role for CDK5RAP2 in vertebrate cells. By disrupting two evolutionarily c...
متن کاملRHAMM is a centrosomal protein that interacts with dynein and maintains spindle pole stability.
The receptor for hyaluronan-mediated motility (RHAMM), an acidic coiled coil protein, has previously been characterized as a cell surface receptor for hyaluronan, and a microtubule-associated intracellular hyaluronan binding protein. In this study, we demonstrate that a subset of cellular RHAMM localizes to the centrosome and functions in the maintenance of spindle integrity. We confirm a previ...
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ژورنال
عنوان ژورنال: Journal of Cell Science
سال: 2017
ISSN: 1477-9137,0021-9533
DOI: 10.1242/jcs.203703